Could dried blood stains provide important information in longitudinal studies?



A study, published in Fast communications in mass spectrometry, tested the efficacy of serum samples against dried blood stains (DBS), analyzing overlapping lipids and polar metabolites. The team wanted to determine how useful DBS might be in longitudinal studies following metabolic disease.

The collection of biofluids plays an important role in longitudinal studies of the disease and because serum blood samples should be collected by a professional and stored at -80 ° C, DBS is advantageous as it can be collected by non -professional, they take up less space and can be stored at room temperature.

Researchers at the Pacific Northwest National Laboratory (WA, USA) used case-control samples collected in 2000-2001 from affected male patients; High body mass index, glucose levels, triglycerides and low density lipoproteins. These samples were compared to a control group of men with normal levels.

The team stored the DBS samples at room temperature, which were then compared to serum samples stored at -80 ° C. A total of 400 lipids and polar metabolites were identified from the samples by mass spectrometry. Metabolites and lipids detected in DBS were compared with those in serum samples to determine the occurrence of molecular degradation over time.

From the results, it was shown that lipids could be analyzed in older DBS samples, providing longitudinal studies with important data. However, it was also concluded that future studies are needed to establish the efficiency of metabolite and lipid analysis in fresh DBS and serum samples, and in DBS samples that have been stored at temperatures. cooler for a long time.

Sources: Kyle JE, Casey CP, Stratton KG et al. Comparison of identified and statistically significant lipids and polar metabolites in 15-year-old serum and dried blood samples for longitudinal studies. Fast Common. Mass spectrum. 31 (5), 447-456 (2017);



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